Image segmentation has now become one of the indispensable steps in digital image processing. Due to various image sources and target forms, no one method is suitable for all images. In order to separate the branches and crossing and overlapping roots, this paper puts forward a method based on the combination of morphology and distance transformation.Morphology is used to dilate intersections of roots skeleton and get root segments. Improved distance transformation is used to get influence areas and the bou...