【摘要】 目的:改善再生丝素蛋白的降解性及力学性能,评价丝素蛋白/聚己内酯纳米纤维支架神经生物材料的生物相容性。方法:采用静电纺丝技术制备丝素蛋白/聚己内酯纳米纤维支架。体外培养雪旺细胞并与支架及其浸提液共培养,通过荧光染色,细胞毒性试验(MTT法)检测其细胞生物相容性。将纤维支架材料在体外置于蛋白酶ⅪV溶液评价其体外降解行为;通过皮下埋植实验观察纤维材料在体内的局部组织反应。结果:丝素蛋白/聚己内酯支架材料,呈现三维网状结构。雪旺细胞具有良好的生长形态;无细胞毒性。随着丝素蛋白比例的降低,能够显著增加混合支架的降解速度。皮下移植实验未引起明显免疫排斥反应,炎症反应轻。结论:丝素蛋白/聚己内酯支架具有良好的生物相容性和生物可降解性,有望用于神经组织工程支架材料修复神经缺损。更多还原

【Abstract】 Objective:To improve the degradation and mechanical behaviors of regenerated silk fibrion and evaluate the biocompatibility of electrospun silk fibroin/polycaprolactone(PCL) nanofiber scaffolds.Methods:The silk fibroin/PCL nanofiber scaffolds were fabricated by electrospinning.MTT assay was used to investigate the proliferation of Schwann cells in the scaffold extracts,and the cytoskeleton of Schwann cells on the scaffold was analyzed by FITC-phalloidin and DAPI staining.The degradation behaviors of the silk fibroin/PCL scaffolds were evaluated in the protease XIV solution.Histologic analysis was conducted to estimate the biocompatibility of the scaffolds in vivo.Results:The electrospun silk fibroin/PCL nanofibers exhibited porous three-dimensional structure.Schwann cells proliferated very well and maintained their normal configuration.The degradation rate of silk fibroin/PCL blend nanofibers gradually increased with the decreasing ratio of silk fibroin to PCL.The subcutaneous transplantation experiment did not cause the obvious immune and inflammatory response.Conclusion:The electrospun silk fibroin/PCL scaffolds have good biocompatibility and well-controlled degradation rate.It is a potential biomaterial to prepare tissue engineered nerve graft for repairing peripheral nerve defect.更多还原