【摘要】 目的:探讨外源性神经生长因子(NGF)对APP/PS1转基因小鼠Wnt/β-catenin信号通路的调节。方法:鼻腔给予小鼠NGF治疗14周,应用免疫组织化学检测小鼠脑内β淀粉样蛋白(Aβ)老年斑的分布,应用免疫印迹检测小鼠脑内糖原合酶激酶3β(GSK3β)、p-GSK3β、β-连环蛋白(β-catenin)和p-β-catenin的表达。结果:Aβ免疫组织化学显色结果显示,与对照组小鼠大脑内的Aβ老年斑相比较,NGF治疗组转基因小鼠大脑内的Aβ老年斑数量减少了30.22%,沉积减少了35.85%,差异有统计学意义。免疫印迹结果显示,外源性NGF减少APP/PS1转基因小鼠脑内GSK3β的表达,增加β-catenin的表达。结论:外源性NGF可能通过抑制GSK3β的活性和增加β-catenin的活性,激活Wnt/β-catenin信号通路,对神经元起保护作用。更多还原

【Abstract】 Objective:To study the regulation of exogenous nerve growth factor(NGF) on Wnt/β-catenin signaling pathway in the APP/PS1 transgenic mica Methods:The mice were treated with NGF via the nasal cavity for 14 weeks.Immunohistochemistry was applied to examine the distribution of Aβ plaques in the APP/PS1 mice brains.Western blotting was used to test the expressions of GSK3β,p-GSK3β,β-catenin and p-β-catenin in the mice brains.Results:Immunohistochemical staining showed that compared with the control group,the number of Aβ senile plaques in the NGF treatment mice brains was reduced by 30.22%and the deposition of senile plaques was decreased by 35.85%.Western blotting showed that exogenous NGF reduced the expressions of GSK3β and increased the expressions of β-catenin in the APP/PSl transgenic mice brains.Conclusion:Exogenous NGF has a neuroprotective effect on the APP/PS1 transgenic mice by activating the Wnt/β-catenin signaling pathway.更多还原