【摘要】 从福建某五金厂综合污水中筛选到一株对Ni2+耐受性400 mg/L的菌株,对其形态学、16S r DNA序列及生理生化特征进行分析,并利用较佳生长条件对所得菌株进行Ni2+吸附验证.结果表明:该菌为革兰氏阴性杆菌,在牛肉膏蛋白胨培养基上形成的菌落,表面较光滑,边缘整齐;经16S r DNA序列比对及生理生化特征的分析,将菌株JMUZJ-1鉴定为寡养单胞菌(Stenotrophomonas sp.);菌株JMUZJ-1生长的较好条件是p H 7.0,温度30℃,Na Cl质量分数0.5%,在该条件下,菌株对Ni2+表现出良好的吸附性能.更多还原

【Abstract】 A nickel resistant strain was obtained from a hardware factory in Fujian province by selection of spontaneous mutants in wastewater containing Ni2 +. Morphological tests,molecular identifications of 16 S r DNA and biochemical characterization to this strain have been performed. The efficiency of the strain in absorbing Ni2 +under optimal growing conditions has been analyzed. The results showed that the nickel-resistant strain was Gram-negative. The strain produces yellow pigmented colonies on medium including beef extract and peptone,with smooth surface and neat edge. Sequence analysis of 16 S r DNA and biochemical characteristics identified the strain JMUI- 1 as Stenotrophomonas sp. The optimal growth condition for the nickel resistant strain is at p H 7. 0,30 ℃,containing 0. 5% Na Cl solution,which allows JMUI- 1 a good absorption performance of Ni2 +.更多还原