【摘要】 克隆得到了调节雌雄激素平衡的大黄鱼cyp19a/b基因,cyp19a基因c DNA全长1805 bp(NCBI登录号:FJ800566),其中开放阅读框1557 bp,编码518个氨基酸;cyp19b基因c DNA全长2268 bp(NCBI登录号:FJ800567),其中开放阅读框1503 bp,编码500个氨基酸.荧光定量PCR分析显示cyp19a基因在性腺中有明显的表达,且在卵巢中的表达量极显著且高于精巢;在肌肉、脑、肝脏、肾脏、脾脏等组织器官中基本不表达.而cyp19b基因在脑、脾脏和肝脏中有较高表达,在性腺和其他器官中基本不表达.更多还原

【Abstract】 Gonad development is dependent on balance between estrogen and androgen,which is regulated by expression of the cyp19 gene and its product aromatase cytochrome P450. Two cyp19 genes named as cyp19 a and cyp19 b have been identified in fish. In this study,both the cyp19 a and cyp19 b have been cloned from Larimichthys crocea. The cDNA of cyp19 a gene is 1805 bp in length( NCBI: FJ800566),including the1557 bp open reading frame( ORF) which codes a polypeptide of 518 amino-acid. The cDNA of cyp19 b gene is 2268 bp in length( NCBI: FJ800567),including the 1503 bp ORF which codes a polypeptide of 500 amino-acid. Real-time PCR results show that the expression level of cyp19 a in ovary is significantly higher than in testis. And the expression level of cyp19 a in gonad is higher than other tissues. The expression level of cyp19 b gene in brain,spleen and liver is higher than in gonad,muscle,and kidney.更多还原