【摘要】 目的研究建立刺头复叶耳蕨中总黄酮的含量测定方法。方法利用紫外可见分光光度法(比色法),以芦丁为标准品,测定标准曲线,得到线性回归方程,对测定方法进行考察。结果以芦丁为标准品,建立了标准曲线的回归方程为Y=0.0121X-0.0322,R2=0.9998,线性关系在17.20-68.80μg/m L范围内。总黄酮平均回收率为101.36%,RSD为0.824%(n=6)。结论该方法操作简单,方法精密度、重现性良好,样品溶液在40min稳定性良好,可用于刺头复叶耳蕨中总黄酮含量的测定,为质量控制提供依据。更多还原

【Abstract】 OBJECTIVE To establish ultraviolet spectrophotometry for the content determination of total flavones from Arachniodes Exilis Ching. METHODS Total flavones in Arachniodes Exilis Ching were determined by ultraviolet spectrophotometry with rutin as the reference substance. RESU LTS The regression equation of rutin standard curve:Y = 0. 0121X- 0. 0322 was established,the correlation coefficient is 0. 9998. The linear range for rutin was 17. 20- 68.80μg / m L. The recovery rate of total flavones was 101. 36%,RSD = 0. 824%( n = 6). CONCLU SION This method was fit to determine the total flavonids of Arachniodes Exilis Ching for the quality control,and it was easy to handle with good credibility,repeatability and stability.更多还原