【摘要】 建立薄层色谱(TLC)与表面增强拉曼光谱(SERS)联用法快速分析复方杜仲胶囊中非法添加的化学药物。利用TLC以甲苯-乙酸乙酯-乙醇-浓氨水(体积比为12∶3∶3∶0.2)为展开剂,实现模拟阳性药中添加成分的初步分离,再采用SERS分析被分离的化学药物斑点。通过TLC-SERS联用法获得的复方杜仲胶囊中各添加化学药物与各对照品的拉曼光谱图特征峰基本一致。TLC-SERS联用法可快速、准确、灵敏地检测出复方降压类中成药中所添加的化学药物。更多还原

【Abstract】 A rapid method with thin layer chromatography(TLC) combined with surface enhanced Raman spectroscopy(SERS) for detection of the chemical drugs illegally added into compound product of Eucommiae capsule is developed. Chemical drugs added in simulation of positive drugs are separated by TLC initially, using toluene-ethyl acetate-ethanol-strong aqua(12∶3∶3∶0.2) as the mobile phase, and then the SERS method is used to analyze the separated chemical drug spots. The characteristic peaks of Raman spectra of the chemical drugs added into the compound product of Eucommiae capsule obtained by TLC-SERS method are nearly consistent with the reference substance. This TLC- SERS method can be used for detection of chemical drugs illegally added into compound anti-hypertensive Chinese patent medicine quickly, accurately and sensitively.更多还原