【摘要】 目的观察L-谷氨酰胺对非酒精性脂肪肝病大鼠的作用。方法将大鼠随机分为对照组、高脂饮食组及L-谷氨酰胺治疗组,高脂饮食组与L-谷氨酰胺治疗组用高脂饮食建立非酒精性脂肪肝病模型,造模成功后给予相应治疗,8周后处死大鼠并检测相关指标。结果治疗组相较于高脂饮食组的炎性反应明显减轻,脂肪空泡减少;治疗组的LDL、AST、TBA、CHOL、TG、LDL-C、IL-6、TNF-α、IL-α、IL-β、MDA及CRP较高脂饮食组明显降低(P<0.05),GSH、T-AOC、HDL-C明显升高(P<0.05),SREBF1和ACCαmRNA与蛋白表达明显下降(P<0.05),PPARαmRNA及蛋白表达明显升高(P<0.05)。结论 L-谷氨酰胺可缓解大鼠非酒精性脂肪肝病症状,其机制可能是通过降低SREBF1及ACCα表达、同时增加PPARα表达实现的。更多还原

【Abstract】 Objective To observe the effect of L-glutamine on fat-induced nonalcoholic fatty liver disease( NAFLD) in rat. Methods Rats were randomly divided into control group,model group and L-glutamine treatment group. Build nonalcoholic fatty liver disease model after the high-fat diet in model group and L-glutamine treatment group. The appropriate treatment was given in modeling,rats were sacrificed after 8 weeks treatment to detect relevant indicators. Results Compared with the model group,liver pathology profile in treatment group alleviated,fat vacuoles reduced; LDL,AST,TBA CHOL,TG,LDL-C,IL-6,TNF-α,IL-α,IL-β,MDA and CRP was significantly lower( P < 0. 05); GSH,T-AOC,HDL-C was significantly higher( P < 0. 05); SREBF1 and ACCα mRNA and protein expression was significantly decreased( P < 0. 05),PPARα mRNA and protein expression was significantly increased( P < 0. 05). Conclusions L-glutamine can alleviate the symptoms of nonalcoholic fatty liver disease in rats,the mechanism may be reducing SREBF1 and ACCα expression,whilepromoting PPARα expression achieved.更多还原