【摘要】 目的对北京市昌平区2011 2014年分离获得的74株食源性金黄色葡萄球菌耐药谱、毒力因子编码基因携带情况和分子流行病学特征进行研究分析,为金黄色葡萄球菌食源性疾病的预防控制提供参考。方法依据GB 4789.10-2010进行菌株分离和鉴定,用全自动微生物鉴定及药敏分析系统VITEK2 Compact进行16种药物敏感性试验,并使用检测试剂盒进行β-内酰胺酶的检测;利用PCR方法检测分离菌株肠毒素,杀白细胞素和耐热磷酸酶等毒力因子编码基因;脉冲场凝胶电泳(PFGE)方法对分离株进行分子分型。结果 74株金黄色葡萄球菌中68株(91.89%)有不同程度的耐药,其中对青霉素耐药率(87.84%)最高,其次为红霉素(44.59%)和克林霉素(39.19%)。毒力因子检测结果显示肠毒素阳性率64.86%(48/74),产2种以上肠毒素菌株占72.92%(35/48)。耐热磷酸酶阳性率89.19%,杀白细胞素编码基因luk F阳性率77.03%。PFGE分型结果显示除9株菌不可分型外,其余65株菌分为40个不同型别,无优势型别。结论昌平区2011 2014年分离的食源性金黄色葡萄球菌具有较强的耐药性、分子多态性和毒力因子携带率较高的特征,说明这些菌株可以导致潜在的食品安全问题。更多还原

【Abstract】 Objective To understand the drug susceptibility, virulence genes and molecular epidemiological characteristics of 74 strains of Staphylococcus aureus isolated in Changping district of Beijing from 2011 to 2014,and provide evidence for the control and prevention of food-borne infection caused by S. aureus. Methods The strains were isolated and identified according to GB 4789. 10- 2010. The resistance to 16 antibiotics was tested with VITEK2 Compact system,β- lactamase was detected with specific kit. PCR was used to detect the genes encoding enterotoxin,leukocidin,and heat-stable phosphatase. Pulsed field gel electrophoresis( PFGE) was used for the molecular subtyping of the strains. Results Sixty eight strains( 91. 89%) were antibiotic resistant,and the resistant rate to penicillin was highest( 87. 84%),follow ed by the resistant rates to erythromycin( 44. 59%) and clindamycin( 39. 19%). For virulence gene detection,the positive rate of enterotoxin genes was 64. 86%,and 35 strains( 72. 92%) produced ≥2kinds of enterotoxin,the positive rate of heat-stable phosphatase was 89. 19%; and the positive rate of luk F gene encoding leukocidin was 77. 03%. Sixty five strains belonged to 40 PFGE patterns,and 9 strains could not be digested by restricted enzyme. Conclusion The strains of S. aureus isolated in Changping were highly antibiotic resistant,molecular polymorphism was found among them,and detection rate of virulence genes was high,indicating the high risk to cause food borne infection.更多还原