【摘要】 目的研究中药雄黄主要成分As2S2对人肝癌细胞株QGY-77 03增值和凋亡的影响及其可能机制。方法 2012年9月—2013年4月,分析不同浓度的As2S2处理QGY-7703细胞后,MTT法测定细胞增殖抑制率;流式细胞仪检测细胞凋亡率;免疫细胞化学方法检测PCNA的表达情况。结果较低浓度(7.5 mg/L)的As2S2对QGY-7703细胞的生长无影响;15~120mg/L的As2S2可抑制细胞的生长,具有时间依赖性和浓度依赖性(P<0.01)。As2S2在15~30 mg/L范围内,细胞凋亡率随浓度和作用时间增加而增加(P<0.01);60 mg/L组凋亡率比低浓度组低,表现为继发性坏死细胞增多。As2S2处理组可明显降低PCNA蛋白的阳性表达(P<0.01)。结论 As2S2在一定的浓度范围内可以诱导QGY-7703细胞发生凋亡,通过下调PCNA的表达抑制细胞的增殖,随着作用时间的延长和(或)浓度的提高,As2S2表现出一定的细胞毒作用,促进肿瘤细胞坏死。更多还原

【Abstract】 Objective To study the effect of realgar main component As2S2 on the proliferation and apoptosis of human hepatocellular carcinoma cell line QGY-7703 and the possible mechanism. Methods From September 2012 to April 2013 QGY-7703 cell was treated by different concentrations of As2S2 first, then the cell proliferation inhibition rate of QGY-7703 cell was detected by MTT method; the cell apoptosis was tested by flow cytometry; the expression of PCNA was detected by immunocytochemistry method. Results As2S2 at low concentration(7.5mg/L) had no effect on the growth of QGY-7703 cells; As2S2 with the concentration of 15mg/L~120mg/L could inhibit the growth of QGY-7703 cells, the inhibitory rate depended on the concentration and time(P<0.01). As2S2 with the concentration of 15 mg/L~30 mg/L, the cell apoptotic rate increased with the increase of concentration and action time(P<0.01). The cell apoptotic rate of 60 mg/L group was lower than those of low concentration groups, and the secondary necrotic cells increased. The positive expression level of PCNA protein of As2S2 group decreased significantly(P<0.01). Conclusion As2S2 in a certain range of concentration can induce the apoptosis of QGY-7703 cells, inhibit the proliferation of the cells by down-regulation of the expression of PCNA. And as the longer duration of action and(or) the increase of the concentration, As2S2 showed certain cytotoxic effect, promoting tumor cell necrosis.更多还原