【摘要】 目的采用组织芯片分析PRSS2蛋白在胃癌组织中表达与意义,为获取胃癌诊断相关的蛋白分子提供实验资料。方法首先收集8例胃癌手术切除标本,包括正常胃粘膜、癌旁组织与胃癌组织,选用Westernblotting方法检测PRSS2蛋白在正常胃粘膜、癌旁组织与胃癌组织中的表达;其次运用免疫组织化学染色方法观察组织芯片(包括正常胃粘膜、癌旁和胃癌组织)中PRSS2蛋白在胃癌组织中的表达,分析其临床意义。结果胃癌组织中PRSS2蛋白表达较正常胃粘膜和癌旁组织明显升高,癌旁组织中的表达较正常胃粘膜组织升高;正常胃粘膜、癌旁和胃癌组织中PRSS2蛋白的阳性表达率分别为14.29%(5/34)、26.92%(7/26)和77.78%(63/81),在胃癌组织中高分化、中分化和低分化腺癌的阳性表达率分别为60.00%(3/5)、70.59%(12/17)和81.36%(48/59);PRSS2蛋白的阳性表达与胃癌组织的分化程度也呈现负相关。结论 PRSS2蛋白在胃癌组织中表达较正常胃粘膜和癌旁组织高,癌旁组织中的表达较正常胃粘膜组织高,该蛋白表达与胃癌组织的发生和分化程度有关。更多还原

【Abstract】 Objective The significance and expression of PRSS2 protein was analyzed by tissue array in gastric cancer tissue and provide valuable data for finding diagnosis related protein of gastric cancer. Methods The 8 cases including normal gastric mucosa,cancer side tissue and gastric cancer were respectively collected. The expression of PRSS2 proteins was checked by Western-blotting. Then,the expression of PRSS2 protein was detected in gastric tissue arrays including normal gastric mucosa,cancer side tissue and gastric cancer by immunohistochemistry staining. The clinical pathology significance of expression of PRSS2 protein was analyzed in gastric cancer tissue. Results The expressions of PRSS2 protein was higher in gastric cancer tissue than that in normal gastric mucosa and cancer side tissue. It was higher in cancer side tissue than that in normal gastric mucosa.The expression positive rates of PRSS2 protein were 14. 29%( 5 /34),26. 92%( 7 /26) and 77. 78%( 63 /81) in normal gastric mucosa,cancer side tissue and gastric cancer tissue,respectively. The expressiong of PRSS2 was 60. 00%( 3 /5),70. 59%( 12 /17) and 81. 36%( 48 /59) in gastric well differentiated,middle differentiated and poorly differentiated adenocarcinoma,respectively.( P < 0. 01). There are negative relation between tissue differentiation level of gastric cancer and the expression positive rate of PRSS2( P < 0. 01). Conclusion The expression positive rate of PRSS2 protein was higher in gastric cancer tissue than that in normal gastric mucosa and cancer side tissue and higher in cancer side tissue than in normal gastric mucosa tissue. The expression of PRSS2 protein was related with effluence and differentaiation level of gastric cancer tissue.更多还原