【摘要】 目的制备Flavokawain A脂质体并考察其理化性质。方法采用薄膜分散法制备Flavokawain A脂质体,在单因素考察的基础上,采用正交实验设计优化最佳处方和工艺;纳米粒度仪测定其粒径和电位分布,葡聚糖凝胶柱法分离脂质体与未包裹的药物,HPLC法测定包封率和载药量,透析法探讨其体外释放行为。结果通过单因素和正交实验,筛选出制备FKA脂质体的最佳处方为磷脂180mg,胆固醇60mg,FKA3mg,37℃成膜1h,55℃水化1.5h,300W超声90秒,制备的FKA脂质体的平均粒径为153.28±11.36nm,平均电位为0.42±0.11m V,平均包封率为(87.57±5.49)%,FKA脂质体在体外约48h释放80%,为原料药的释放时间的4倍。结论采用薄膜分散法制备的Flavokawain A脂质体,包封率高,稳定性好,且具有一定的缓释性。更多还原

【Abstract】 Objective To prepare Flavokawain A liposomes,and study its chemicophysical property. Methods The film dispersion method was use to prepare Flavokawain A liposomes,at the base of single factor study,the best prescription and the preparation conditions was setting by by orthogonal test,the size and zeta value was tested,the EE% was determined by HPLC,the releasing rate was test by the method of the dialysis. Results The optimal prescription of FKA liposomes preparation is lipid 180 mg,cholesterol 60 mg,FKA 3mg,rotary evaporate at 37℃ for 1 hour,and then hydrated at 55℃ for 1. 5hour,at last transonic at 300 W for 90 s. The average size of FKA liposomes was( 153. 28 ± 11. 36) nm,zeta potential of mean( 0. 42 ± 0. 11) m V; its entrapment efficiency reached as high as( 87. 57 ± 5. 49) %. The FKA liposomes release 80% in PBS need 48 h which was 4 tines of FKA release. Conclusion Use film dispersion method to prepare Flavokawain A Liposomes has high entrapment efficiency,good stability and sustained release.更多还原