目的建立不同产地铁包金药材的HPLC指纹图谱。方法色谱柱为Boston C18(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-0.1%磷酸溶液,梯度洗脱,流速1.0 m L·min-1,检测波长260 nm,柱温37℃。结果建立了铁包金药材的指纹图谱,确定了12个共有特征峰,标定了其中的2个峰,并对不同产地铁包金的指纹图谱进行了相似度比较。结论所用方法可用于铁包金药材的质量评价。
【英文摘要】
OBJECTIVE To establish an HPLC fingerprint analysis method for Berchemiae lineatae Radix from the different habitats.METHODS The HPLC analysis was carried out on a Boston C18( 250 mm × 4. 6mm,5 μm) chromatographic column with mobile phase of methanol- acetonitrile- 0. 1% phosphoric acid in gradient elution. The flow rate was 1. 0 m L·min- 1with the ultraviolet detection wavelength of 260 nm and the column temperature of 37 ℃. RESULTS The fingerprints of B. lineatae Radix from the different habitats were est...