【摘要】 目的研究绿原酸(CGA)对肝星状细胞增殖、胶原蛋白(Collagen)Ⅰ、CollagenⅢ、基质金属蛋白酶抑制剂-1(TIMP-1)及基质金属蛋白酶-2(MMP-2)表达及分泌的影响。方法体外培养大鼠肝星状细胞株HSCT6,随机分为5组:阴性对照组、血小板衍生生长因子(PDGF)组、PDGF+CGA(12.5μg/mL)组、PDGF+CGA(25μg/mL)组和PDGF+CGA(50μg/mL)组。干预24h后,MTT法检测HSC-T6的增殖情况;RT-PCR检测CollagenⅠ、CollagenⅢ、TIMP-1及MMP-2 mRNA表达;ELISA检测细胞上清液中CollagenⅠ、CollagenⅢ、TIMP-1及MMP-2的蛋白含量。结果 CGA可明显抑制PDGF诱导的肝星状细胞增殖(P<0.05),且随着剂量的增加,抑制作用逐渐增强(P<0.05);CGA可明显抑制PDGF诱导的CollagenⅠ、CollagenⅢ、TIMP-1的表达和分泌(P<0.05),但对MMP-2的表达和分泌无明显影响。结论 CGA可通过抑制肝星状细胞增殖及细胞外基质合成、促进细胞外基质降解来发挥抗肝纤维化作用。更多还原

【Abstract】 Objective To investigate the effects of chlorogenic acid(CGA)on hepatic stellate cell proliferation and the expression and secretion of Collagen Ⅰ,Collagen Ⅲ,tissue inhibitors of metalloproteinase-1(TIMP-1)and matrix metalloproteinase-2(MMP-2).Methods An immortalized rat hepatic stellate cell(HSC)line was cultured in vitro.The cells were divided into 5groups:control group;platelet-derived growth factor(PDGF)(10ng/mL),PDGF+CGA(12.5μg/mL),PDGF+CGA(25μg/mL),PDGF+CGA(50μg/mL)and CGA(50μg/mL)group.After 24 hours treatment,the proliferation of HSC was detected by MTT method.The mRNA expression of CollagenⅠ,CollagenⅢ,TIMP-1and MMP-2were detected by RT-PCR.The protein levels of Collagen Ⅰ,Collagen Ⅲ,TIMP-1and MMP-2in the culture supernatant of HSC were measured by ELISA.Results PDGF increased the hepatic stellate cell proliferation,the mRNA expression and the protein levels of Collagen Ⅰ,Collagen Ⅲ and TIMP-1(P <0.05),which were significantly decreased by CGA(P <0.05).However,CGA had no significant influence on the expression of MMP-2.Conclusion The antifibrotic effect of CGA may be related with the inhibition of hepatic stellate cell proliferation and generation of extracelluar matrix and promotion of extracelluar matrix degradation.更多还原