【摘要】 建立了微芯片毛细管电泳非接触电导检测法快速测定盐酸倍他洛尔滴眼液中盐酸倍他洛尔的含量。探讨了缓冲液类型、浓度、分离电压及进样时间等因素对分离检测的影响。实验采用1.5 mmol·L-1HAc-1.5mmol·L-1Na Ac(p H=4.69)为缓冲溶液,分离电压为2.1 k V,进样时间10.0 s。此条件下于0.7 min内实现了盐酸倍他洛尔的快速分离测定。盐酸倍他洛尔的浓度在5.0~200.0μg·m L-1线性良好(r=0.9997,n=6),检出限为1.0μg·m L-1(S/N=3),RSD为0.8%,样品的加标回收率为100.4%~102.0%。滴眼液中的辅料在该条件下不干扰测定,可成功测定盐酸倍他洛尔滴眼液中盐酸倍他洛尔的含量。更多还原

【Abstract】 A method was developed for the rapid determination of betaxolol hydrochloride in betaxolol hydrochloride eye drops by microchip capillary electrophoresis with contactless conductivity detection. Effects of the type and concentration of buffer,separation voltage and injection time on the separation and detection were investigated. Betaxolol hydrochloride was separated and detected in the buffer solution of 1. 5 mmol·L-1acetic acid-1. 5 mmol·L-1sodium acetate( p H = 4. 69) at a separation voltage of 2. 1 k V within0. 7 min. The peak height showed a good linear relationship with the concentration of betaxolol hydrochloride ranged from 5. 0 μg·m L-1to 200. 0 μg·m L-1,the limit of detection reached 1. 0 μg·m L-1( S/N = 3),the RSD was 0. 8% and the recoveries were100. 4% ~ 102. 0%. The method was used for the analysis of betaxolol hydrochloride in betaxolol hydrochloride eye drops satisfactorily.更多还原