Marcapto-silica microspheres(SiO2-SH MSs)were prepared through a solvothermal route,then these SiO2-SH MSs were further modified by conjugating iminodiacetic acid(IDA)toafford SiO2-SH/IDA MSs.These-SH groups and-IDA groups as binding sites can chelate Ni 2+ions,which can be further used to enrich and separate His-tagged proteins directly from the mixture of lysed cells without sample pretreatment.Results show that these MSs present negligible nonspecific adsorption and high protein binding,which are especia...
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