【摘要】 采用改进无皂乳液聚合法制备了单分散聚苯乙烯微球(PS),并以之为种球,芹菜素(API)为模板分子,2-乙烯基吡啶(2-Vpy)为功能单体,通过单步种子溶胀聚合法制备得到了单分散芹菜素分子印迹微球(API-MIPM)。采用静态和动态结合实验以及选择性实验考察了API-MIPM对底物API的结合特性、吸附动力学及分子识别性能。结果表明,API-MIPM对API的选择性好,吸附容量大,分子识别能力强。API-MIPM对API的静态分配系数KD(6.07mL/g)明显大于其他对照物,对API的吸附量达16.25μmol/g;API-MIPM中存在对API高低不同亲和力的两类结合位点,其中高低亲和力结合位点的离解常数分别为0.087和1.405μmol/mL,最大表观结合量分别为9.17和25.75μmol/g,其对API的吸附行为可用准二级吸附动力学模型进行描述。更多还原

【Abstract】 Monodisperse micrometer-sized polystyrene(PS)microspheres were prepared by improving the formulas and conditions of emulsifier-free polymerization.Apigenin monodispersed molecularly imprinted polymer microspheres(API-MIPM)were prepared by a single-step swelling and polymerization method using the PS microspheres as seeds,apigenin(API)as template molecules and 2-vinylpyridine(2-Vpy)as functional monomer.The adsorption ability and molecular selectivity of API-MIPM were evaluated by static and dynamic equilibrium adsorption experiments and selectivity test experiments.The results revealed that API-MIPM displayed strong affinity,high adsorption capacity and excellent selectivity toward apigenin.The distribution coefficient of API-MIPM for API(6.07mL/g)is higher in respect to competition species,and the adsorption amount of API-MIPM for API is 16.25μmol/g.Furthermore,two kinds of binding sites are formed in the API-MIPM with dissociation constants of 0.087 and 1.405μmol/mL,and the maximum apparent binding capacities are 9.17 and 25.75μmol/g,respectively.The pseudo-second order equation is fitted for absorption of API on the API-MIPM.更多还原