With the in-depth study of gene therapy, the gene transfection techniques have achieved rapid development. Since it was proposed in 1997,reverse transfection concept has also aroused considerable attention.Reverse gene transfection is also known as surface-mediated transfection or matrix-mediated transfection,or solidphase transfection. In a typical procedure of reverse transfection,DNA( or RNA) is firstly anchored to the substrate; cells are transplanted on the substrate surface in the next step; the adsor...