Objective: To separate and purify endophytic fungi from different organs of Rabdosia rubescens( Hemsl.) Hara. by using tissue separation method to determine the optimal separation culture medium and types of endophytic fungi. Methods: Different organs( leaf,stem) in Rabdosia rubescens( Hemsl.) Hara. were used for experiment materials to separate and purify its endophytic fungi to a single colony by using tissue separation method. Then they were set aside for future use. Results: The total 256 endophytic fun...