【摘要】 目的考察盐酸青藤碱对雄性大鼠精子质量、睾丸组织形态及精子顶体酶、睾丸乳酸脱氢酶活性的影响。方法 SD雄性8周龄大鼠36只,随机分成空白对照组、盐酸青藤碱低剂量组(60 mg/kg)、盐酸青藤碱高剂量组(240 mg/kg),连续给药10周。观察各组大鼠精子密度、活动度、畸形率、活精率、精子顶体酶、睾丸乳酸脱氢酶活性以及睾丸组织形态。结果与空白对照组比较,盐酸青藤碱使附睾质量系数下降(P<0.05);对大鼠精子密度、畸形率、活动度、活精率、精子顶体酶活性无明显影响(P>0.05);高、低剂量升高睾丸乳酸脱氢酶活性的作用均有显著性(P<0.01,P<0.05);对睾丸组织形态无明显改变。结论盐酸青藤碱在等效量3倍、12倍时对精子密度、畸形率、活动度、活精率、精子顶体酶、睾丸组织形态均未见明显不良影响。更多还原

【Abstract】 Objective To investigate the effect of sinomenine hydrochloride on sperm quality and testicular tissue morphology, sperm acrosome enzyme and testis lactate dehydrogenase( LDH) activity. Methods 36 male 8-week- old SD rat s were randomly divided into the blank control group, sinomenine hydrochloride low- dose group( 60 mg / kg), sinomenine hydrochloride high- dose group( 240 mg / kg), continuous medication for 10 weeks. The sperm density, mobility, deformity rate, live sperm rate, sp erm acrosome enzyme activity, testis LDH activity and testicular tissue form in each group rats were observed. Results Compared with the blank control group, the body weight index of epididymis was decreased(P <0.05); There were no statistical difference in sperm density, sperm malformation rate,sperm motility rate, living sperm rate and sperm acrosome enzyme activity(P >0.05); The LDH activity of testicular tissue in high and low dose sinomenine groups were obvious significant(P <0.01, P <0.05); There were no differences in each dose group of hydrochloric acid sinomenine on testicular tissue morphology. Conclusion The three times and twelve times equivalent sinomenine hydrochloride didn’t show obvious adverse effect on sperm density, malformation rate, motility rate,living sperm rate, sperm acrosome enzyme and testicular tissue morphology.更多还原