Expression and significance of CD3~+CD16~+CD56~+ natural killer T cells in peripheral blood of patients with unexplained recurrent spontaneous abortion
目的探讨自然杀伤T细胞(natural killer T cell,NKT)在原因不明习惯性流产(unexplained recurrent spontaneous abortion,URSA)发生机制中的作用。方法有URSA史患者50例,其中未孕者20例为URSA非孕组,难免流产者30例为UESA难免流产组,52例正常体检者中正常未生育者20例为正常未育组,正常妊娠孕妇20例为正常妊娠组,有正常生育史12例为正常生育组,采用流式细胞术检测各组外周血中CD3+CD16+CD56+NKT细胞的表达水平。结果外周血CD3+CD16+CD56+NKT细胞百分率正常未生育组[(4.62±1.38)%]与正常生育组[(4.90±3.00)%]比较差异无统计学意义(P>0.05);URSA非孕组外周血CD3+CD16+CD56+NKT细胞百分率[(2.42±1.52)%]低于正常生育组(P<0.05),URSA难免流产组[(4.18±2.77)%]低于正常妊娠组[(14.40±2.27)%](P<0.05);正常妊娠组外周血中NKT细胞百分率是正常生育组的3倍,而URSA难免流产组是URSA非孕组的2倍。结论 URSA患...
【英文摘要】
Objective To investigate the role of natural killer T cell(NKT)in the mechanism of unexplained recurrent spontaneous abortion(URSA).Methods In 50 patients with URSA history,there were 20non-pregnancy patients and30 pregnancy patients.Another 52 healthy volunteers included 20 women with no child-bearing history,20 normal pregnancy women,and 12 women with normal child-bearing history.The flow cytometry was used to detect the expressions of CD3+CD16+CD56+NKT cells in peripheral blood in all groups.Results Ther...
【基金】
广东省江门市科技计划项目(20147140)
【更新日期】
2015-08-14
【分类号】
R714.21
【正文快照】
维持正常妊娠有赖于母胎免疫耐受的形成[1],自然杀伤T细胞(natural killer T cell,NKT)是一类具有自然杀伤细胞受体和T细胞受体且显示自然杀伤细胞和T细胞两方面性质的T细胞亚群。目前多以CD3+CD16+CD56+作为检测NKT细胞的标志。NKT细胞的生物学作用主要包括免疫调节和细胞毒作