【摘要】 目的 :研究腰椎间盘突出患者髓核与纤维环组织中MMP及TIMP的表达,并探讨其在腰椎间盘退变中的意义。方法 :选取腰椎间盘突出症患者切除的椎间盘标本50例,作为实验组,选择同时期突发创伤致腰部椎体骨折手术摘除椎间盘10例作为对照组。分离椎间盘髓核与纤维环,采用免疫组化法检测椎间盘标本中髓核及纤维环细胞中MMP-1、MMP-2、MMP-3、MMP-9、MMP-13、TIMP-1、TIMP-2的表达。结果 :MMPs与TIMPs在腰椎间盘突出患者髓核与纤维环中的表达高于对照组,随着病情的加重MMPs与TIMPs的表达率也显著增高。结论 :MMPs、TIMPs表达失衡可能是基质降解导致腰椎间盘突出发生的重要原因。更多还原

【Abstract】 Objective To observation the expression of MMPs and TIMPs in nucleus and annulus for patients with lumbar disc protrusion, and to explore its significance in lumbar disc degeneration. Methods 50 case of intervertebral disc samples in patients with lumbar disc protrusion were selected as experimental group, and 10 case of intervertebral disc samples in patients with lumbar vertebral fracture by emergency trauma were selected as control group. The express of MMP-1, MMP-2, MMP-3, MMP-9, MMP-13 and TIMP-1, TIMP-2 in nucleus and annulus were detected by immunohistochemistry. Results The express of MMPs and TIMPs in nucleus and annulus in patients with lumbar disc protrusion were all higher than control group, and The express rate of MMPs and TIMPs were significantly increased with the aggravation of the disease. Conclusion The imbalance expression of MMPs, TIMPs maybe the important cause for matrix degradation which lead the occurrence of lumbar disc herniation.更多还原