【摘要】 为开发可监控两系杂交水稻种子纯度的全新标记,通过60Co-γ辐照两系不育系Y58S干种子,筛选获得白色中脉、转绿型白化叶色、紫叶斑点、黄色、淡绿色与条纹白色等多种类型叶色突变体。与其他叶色突变体比较,突变体wml-1的白色中脉突变性状全生育期稳定,且仅在叶片正面的中脉部分表达,不影响苗高和主要农艺性状,配合力和不育特性也与野生型不育系相类似。遗传分析表明,叶片携带白色中脉突变性状受一对隐性基因控制。本研究结果表明,叶片携带白色中脉突变可以作为一种更理想的标记性状,对发展直观方便辅助去杂和省时节本鉴定种子纯度新技术具有重要意义。更多还原

【Abstract】 To explore novel marker used for monitoring the seed purity of two-line hybrid rice. The dry seeds of two-line male sterile rice Y58 S were irradiated by 60- Cobalt gamma rays,various types of leaf color mutants such as white midrib leaves,green-revertible albino,stripe white,purple spot,yellow,light green were isolated. In mutant wml-1white midrib expressed stably during the whole growth,and only in adaxial surface. No significant influences of wml-1on seedling height and major agronomic traits were found,compared to that of other types of leaf color mutants. Similar sterile characteristics and combining ability were observed between the wml-1and the wild type. Genetic analysis indicated that the mutated trait was controlled by a single recessive gene. All results suggested that white midrib leaf could be used as an ideal leaf marker,which can be of great significance to develop new technology for visible and convenient assisted-elimination of the false plants and time-saving and low-costing identification of the seed purity.更多还原