【摘要】 为探索钙离子与油菜胁迫响应机制的关系,利用45Ca放射性示踪剂研究基因Bn TR1(Brassica napus Thermal Resistant gene 1)对油菜钙吸收的影响。试验分析了从Bn TR1转基因油菜和野生型油菜中提取出来的原生质体对钙离子的吸收,比较其差异。结果表明,当胞外钙离子浓度低于0.9mmol·L-1时,转基因型原生质体比野生型原生质体吸收更多的钙离子;当外界钙离子浓度高于1mmol·L-1时,转基因型原生质体却比野生型原生质体吸收的钙离子更少;当胞外钙离子浓度大于30mmol·L-1时,转基因型原生质体吸收的钙离子大大低于野生型原生质体的吸收量;当加入钙离子通道阻塞剂氯化镧且其浓度从5μmol·L-1上升到20μmol·L-1时,转基因型原生质体会出现钙离子吸收峰值。综上,Bn TR1基因通过对细胞膜上钙离子通道更为灵敏和有效的调控,可以提高油菜对钙离子胁迫的耐受性。研究结果对研究Bn TR1基因的作用机制,以及其对油菜耐胁迫能力的影响提供了新的方向。更多还原

【Abstract】 To understand the relationship between Ca2 +and stress response mechanism of rapes,45 Ca was used as a radioactive tracer to first explore the influences of Brassica napus Thermal Resistant gene 1( Bn TR1) on Ca2 +absorption of rapes. The study thoroughly analyzed the Ca2 +absorption of protoplasts separated from the Bn TR1 transgenic rapes and the non-transgenic rapes,and identified the difference between them. The study found that the protoplasts of the transgenic lines can absorb more Ca2 +than the non-transgenic type if extracellular Ca2 +concentration was less than 0. 9mmol·L- 1. However,the reverse phenomenon was observed as the surrounding Ca2 +concentration was more than 1mmol·L- 1. What’s more,much less Ca2 +was absorbed by the transgenic rape protoplasts than the non-transgenic ones,when the extracellular Ca2 +concentration was greater than 30 mmol·L- 1. It’s worth noting that,the peak value of Ca2 +absorption of transgenic protoplasts occurred,when La Cl3 increased from 5 to 20 μmol·L- 1. Bn TR1 could improve plants’ resistance to Ca2 +stress,through more sensitive and powerful control of calcium ions channel in cell membranes. This finding indicated a new direction about the future research on Bn TR1 gene mechanism as well as its impact on the ability of resistance to stress of rapes.更多还原