【摘要】 为了从鸢乌贼卵中获得不含胆固醇的优质动物磷脂,分别研究了原料预处理方法、单因素萃取对胆固醇脱除率的影响,并在单因素试验的基础上,利用正交试验对亚临界R134a萃取技术脱除鸳乌贼卵中胆固醇的工艺进行了优化。结果表明,在对原料进行胶体磨破碎、木瓜蛋白酶酶解和喷雾干燥的预处理后,鸢乌贼卵粉中胆固醇的脱除率比未经过前处理直接冻干得到的鸢乌贼卵粉中胆固醇的脱除率显著提高,亚临界R134a萃取脱除预处理后的鸢乌贼卵粉中胆固醇的最佳条件为:萃取压力6MPa,萃取温度为70℃,动态萃取时间70min。在最佳条件下,预处理后的鸢乌贼卵粉中胆固醇的脱除率可达99.31%。本文为亚临界R134a脱除食品中胆固醇技术的产业化提供理论依据。更多还原

【Abstract】 In order to obtain cholesterol-free phospholipid from Sthenoteuthis oualaniensis eggs,the technique of cholesterol removal from Sthenoteuthis oualaniensis eggs by subcritical R134 a extraction method was studied. Firstly,the effects of pretreatment and parameters including extraction pressure,extraction temperature and extraction time on the removal yields of cholesterol were investigated. The technical parameters were then optimized by orthogonal design. The results showed that after colloid mill homogenizing,papain hydrolyzing and spray drying,the cholesterol yields of the raw material increased remarkably. The optimal extraction conditions for the technique of cholesterol removal from pre-treated Sthenoteuthis oualaniensis eggs by subcritical R134 a were as follows: extraction pressure was 6MPa, extraction temperature was 70℃ and extraction time was 70 min. The yields of cholesterol removal reached 99. 31% under the optimal extraction conditions. The study laid theoretical foundation on industrialization of the technique on cholesterol removal by subcritical R134 a extraction.更多还原