【摘要】 目的研究三氧化二砷（As₂O₃）脂质体对荷瘤大鼠血脑屏障（BBB）的透过率及其对大鼠脑胶质瘤的生长抑制作用。方法应用超声薄膜分散法制备As₂O₃脂质体,检测其包封率;建立大鼠脑胶质瘤模型,分别给予As₂O₃脂质体和As₂O₃,应用原子荧光法检测给药后大鼠脑组织中的砷浓度,以判定其对血脑屏障的透过性,并与给予生理盐水的对照组相比,从电镜、TUNNEL、MRI影像及大鼠的生存状态等方面研究As₂O₃脂质体对C₆胶质瘤的影响。结果 As₂O₃脂质体组砷的血脑屏障透过性明显提高,电镜及TUNEL法检测示肿瘤细胞凋亡率（20.03%）高于其他2组（3.92%、4.09%）;大鼠中位生存时间（70d）优于生理盐水组（21d）和As₂O₃组（23d）。结论 As₂O₃脂质体可以明显提高鼠脑内As₂O₃浓度,并能诱导胶质瘤细胞的凋亡,抑制大鼠脑胶质瘤的生长,从而延长荷瘤大鼠的生存时间。更多还原

【Abstract】 Objective To investigate the permeation rate of As₂O₃liposomes passing blood brain barrier（BBB）in tumorbearing rats and its inhibition effect on the C₆ glioma growth in vivo.Methods The As₂O₃liposomes were developed by film-ultrasonic wave dispersion method and the encapsulation efficiency was performed.Then all rats were intravenously injected by As₂O₃liposomes and As₂O₃respectively after successfully establishing the glioma models in rats.After that,As₂O₃concentration of brain tissue was measured using atomic fluorescence technique to assess permeability of blood brain barrier.Compared with controls injected by normal saline,we studied the effect of As₂O₃liposomes on C₆ glioma from the following aspects including electron microscopy,TUNNEL staining,Magnetic Resonance Imaging（MRI）and the survival condition.Results The As₂O₃liposomes could obviously facilitate permeability of blood brain barrier.The results of electronic microscopic analysis and TUNNEL staining showed that the apoptosis rate（20.03%）of tumor cells in As₂O₃liposomes group was much higher than that in the other two groups（3.92%,4.09%）.As₂O₃liposomes group had longer median survival time of 70 days than 21 days in the saline group and 23 days in As₂O₃group.Conclusion The As₂O₃ liposomes can significantly improve the As₂O₃concentration of rats’ brain,and can induce apoptosis in glioma cells and suppress intracranial tumor growth and prolong the life span of tumor-bearing rats.更多还原