【摘要】 探讨了5-(4-氯苯基)-2-(2-硝基苯甲酰胺基)-1,3,4-噻二唑(TBA)对油菜种子萌发和幼苗生长的影响,以期为TBA在农业上的应用提供理论依据和参考。在水培条件下,采用不同质量浓度(0、6、8、10、12、14 mg/L)TBA浸泡处理油菜种子,并测定了油菜种子的发芽率、发芽势、发芽指数、活力指数,幼苗的根长、茎高、根粗、茎粗,幼苗叶片内的可溶性蛋白质、可溶性糖、叶绿素含量和硝酸还原酶活性以及幼苗根系活力等相关指标。结果表明:TBA质量浓度在6~10mg/L时,油菜种子的发芽率、发芽势、发芽指数、活力指数以及幼苗的根长、茎高、根粗、茎粗随着TBA质量浓度升高而增大,TBA高于10 mg/L时则有所下降,但仍高于对照;同时,叶片中的可溶性蛋白、可溶性糖、叶绿素含量和硝酸还原酶活性以及幼苗根系活力也随着TBA质量浓度的升高而升高,然后随之下降,在10 mg/L TBA时达到最高。因此,TBA能有效促进油菜种子萌发及幼苗生长,且TBA质量浓度为10 mg/L效果最好。更多还原

【Abstract】 The effects of 5-( 4-chlorophenyl)-2-( 2-nitro-benzamido)-1,3,4-thiadiazole( TBA) on rape seed germination and seedling growth were investigated,so as to provide theoretical reference for TBA application in agricultural production. In this study,using hydroponic condition,the rape seeds were soaked with different concentrations( 0,6,8,10,12,14 mg / L) TBA to investigate the germination rate,germination potential,germination index,vigor index,root length,stem height,root diameter,stem diameter,the contents of soluble protein,soluble sugar,and chlorophyll,the nitrate reductase vigor of leaves and the root activity. The results showed that,the germination rate,germination potential,germination index,vigor index,root length,stem height,root diameter and stem diameter respectively enhanced with the TBA concentration increased( 6—10 mg / L); while the TBA concentration was higher than 10 mg / L,the above indicators declined with the increase of the concentration in the range of 10—14mg / L but they were still higher than the control. Meanwhile,the soluble protein,soluble sugar,chlorophyll contents,the nitrate reductase vigor of leaves and the root activity improved along with the TBA concentration from 0 mg / L to 10 mg / L,with their peak values occurring at 10 mg / L,while above 10 mg / L they declined. So the germination and seedling growth of rape seed could be effectively improved by TBA,and the optimal concentration is 10 mg / L.更多还原