【摘要】 为了优化油体提取方法,获得较高纯度的油体,以甘蓝型油菜鼎油杂4号种子为试验材料,在漂浮离心、去污剂洗涤、离子洗脱液洗脱等程序的基础上,增加9 mol/L尿素洗脱、正己烷萃取等步骤进行优化,并探讨了理化因素对油体稳定性的影响。结果表明:优化后的方法提高了离体油体的完整性,获得的油体纯度较高。随着Na Cl浓度的增加,油体的稳定性下降;p H值≤3及p H值≥7时油体稳定分布,p H值为4~6时油体稳定性明显下降;温度对油体的影响较小,不同温度下油体分布相对稳定。在此基础上,利用SDS-PAGE对油体蛋白进行分离,获得了较清晰的蛋白质条带。更多还原

【Abstract】 In order to optimize the extraction method of oil bodiesand obtain higher purity oil bodies,the seeds of Brassica napus L.(Dingyouza 4) was used as the experiment materials,on the basis of the original method of extracting oil bodies,such as flotation centrifugation,detergent washing and ion eluting,twosteps elution through 9 mol/L urea and extraction through n-hexane were increased to optimize the method. The effects of physical and chemical factors on the oil bodies’ stability were explored. The results showed that: the stable and high purity of oil bodies were obtained by the optimization method. The stability of oil bodies decreased with the increase of Na Cl concentration; the oil bodies stably distributed in p H≤3 and p H≥7 and the oil bodies’ stability decreased obviously when p H value was between 4 and 6.The temperature almost had no effects on the oil bodies’ stability,and the oil bodies were quite stable under different temperatures. On this basis,polyacrylamide gel electrophoresis were used to separate the oil body protein,and obtained a clearer protein band.更多还原