【摘要】 [目的]研究环氧丙烯酰胺(GA)对体外培养的R2C细胞生长及孕酮合成的影响。[方法]用0.25、0.5、0.75、1、2、4、6 mmol/L浓度的GA作用于细胞48 h,MTT法获得GA作用浓度的IC25,IC50及IC75。用以上3种浓度的GA刺激细胞48 h,观察细胞形态。GA作用于细胞4 h后,通过彗星试验(Comet Assay)检测细胞DNA的损伤程度;放射免疫法(RIA)测定GA刺激细胞24 h后的孕酮合成量。[结果]GA能抑制R2C细胞活性,其IC25、IC50、IC75分别为0.635、0.872及1.198 mmol/L;3种浓度的GA能不同程度的影响细胞生长形态;作用4 h对R2C细胞DNA有损伤作用;各浓度组作用24 h后均能降低孕酮的合成量。[结论]GA能抑制R2C细胞增长及孕酮合成能力。更多还原

【Abstract】 [Objective] This study aimed to investigate the effects of glycidamide(GA)on growth and progesterone biosynthesis of rat R2 C Leydig cells cultured in vitro.[Method] The R2 C Leydig cells were treated with GA with concentrations of 0.25,0.5, 0.75, 1, 2, 4 and 6 mmol/L respectively for 48 h. The IC25, IC50 and IC75values of GA were all detected by MTT assay. The Leydig cells were treated with GA at concentrations of IC25, IC50 and IC75respectively for 48 h, and then the morphology of Leydig cells was observed. After the Leydig cells were treated with GA for 4 h,the cellular DNA damage was measured by the comet assay technique; and after the Leydig cells were with treated with GA for 24 h, the progesterone biosynthesis amount was detected by radioimmunoassay(RIA). [Result] GA could inhibit the viability of R2 C Leydig cells, and its IC25, IC50 and IC75were 0.635, 0.872 and 1.198mmol/L, respectively. The GA at concentrations of IC25, IC50 and IC75affected the growth and morphology of rat R2 C Leydig cells in varying degrees. The 4-h treatment of GA could significantly damage the DNA of R2 C Leydig cells, and the 24-h treatment of GA at concentrations of IC25, IC50 and IC75all reduced the progesterone biosynthesis amount. [Conclusion] GA could inhibit the growth and progesterone biosynthesis of rat R2 C Leydig cells.更多还原