【摘要】 基于生物信息学方法,发现并验证了与RBP4基因LS性状(litter size)候选分子标记MspⅠRFLP(SNP G1223C)临近的SNP A1027G(SNPs,single nucleotide polymorphisms),并对大白猪和湖南黑猪群体的RBP4多态性与母猪LS性状的相关性进行了研究。结果表明:RBP4基因SNP G1223C在2个群体中均与母猪LS性状显著相关(P<0.05);RBP4基因SNP A1027G在混合群体中与母猪LS性状显著相关(P<0.05);这2个多态性位点构建的单倍型也与母猪LS性状显著相关(P<0.05),其优势单倍型母畜的LS性状值明显优异于单个SNP位点优势基因型的LS性状值。在分子标记辅助育种中采用RBP4单倍型作为LS性状重要候选基因的分子标记比采用单个多态性位点更具价值。更多还原

【Abstract】 Retinol binding protein 4(RBP4), an important candidate gene for traits of sow litter size(LS), plays a key role in vitamin transportation on the maternal-fetal interface during swine pregnancy. Candidates of exonic single nucleotide polymorphisms(SNPs) in gene RBP4 were scanned through bioinformatics analysis, and SNP A1027 G, neighboring to Msp Ⅰ RFLP(SNP G1223C) with molecular marked was validated, and the correlation between LS traits and polymorphisms of RBP4 gene were further analyzed both on the population of Large White and Hunan black. Results exhibited that both RBP4 MspⅠ RFLP and the lately reported SNP A1027 G were significantly correlated with swine LS traits(P<0.05). The average LS traits performance of most of advantage RBP4 haplotypes was better than those of advantage genotypes with single locus on SNPs. It was suggested that the haplotype of RBP4 gene was a more efficient molecular marker for the improvement of swine LS traits than SNP with a single locus.更多还原