【摘要】 目的分析中晚期非小细胞肺癌患者化疗前后外周血中Treg细胞和Th17细胞比例变化及临床意义。方法收集51例中晚期非小细胞肺癌患者(观察组)化疗前后及47例健康对照组外周血标本,应用流式细胞术检测Treg细胞和Th17细胞所占CD4+T细胞的比例,观察Treg和Th17细胞比例在化疗前后的变化及其相关性。结果化疗后缓解组外周血中Th17及Treg细胞比例较化疗前降低(P<0.05),Treg细胞仍高于对照组(P<0.05),Th17细胞比例稍高于对照组(P>0.05)。非缓解组2种细胞的比例较化疗前增高(P<0.05),并明显高于对照组(P<0.05)。无论是化疗前,还是化疗后,非小细胞肺癌患者外周血中Th17与Treg细胞之间均存在正相关关系(r=0.589、0.657,P<0.05)。结论化疗能够降低非小细胞肺癌患者外周血中Th17及Treg细胞比例,对肿瘤免疫功能具有积极意义。更多还原

【Abstract】 Objective To explore the changes of Th17 and Treg cells in the peripheral blood of patients with advanced non-small cell lung cancer before and after chemotherapy,and to observe the clinical significance. Methods Flow cytometry was used to detect the proportion of Th17 and Treg cells in the peripheral blood of 51 patients with advanced non-small cell lung cancer( the observation group) before and after chemotherapy and 47 healthy volunteers( the control group),and the correlation was observed too. Results The proportion of Th17 and Treg cells of the remission group after chemotherapy were all significantly lower than before chemotherapy( P < 0. 05). Compared with the control group,the proportion of Th17 cells of the remission group did not change significantly( P > 0. 05),but the proportion of Treg cells of the remission group was higher significantly( P < 0. 05). The proportion of Th17 and Treg cells of the non-remission group after chemotherapy were all significantly higher than before chemotherapy( P < 0. 05),and were higher than those of the control group( P < 0. 05). The proportion of Th17 cells were positively related to the proportion of Treg cells in the peripheral blood of patients with advanced non-small cell lung cancer before and after chemotherapy( r = 0. 589,0. 657; P < 0. 05). Conclusion Chemotherapy can reduce the proportion of Th17 cells and Treg cells in the peripheral blood of patients with advanced non-small cell lung cancer,and has the positive significance for tumor immune function.更多还原