【摘要】 以红曲米粉中分离纯化的红曲霉为菌种进行液态发酵,生产红曲色素,利用正交试验设计,研究不同碳源、氮源、pH、装液量对红曲霉色价及菌体干重的影响。结果表明:红曲霉最佳发酵工艺条件为以3%玉米粉为碳源、0.02%酵母膏为氮源,调整发酵液初始pH至4.0,装液量125mL/250mL,红曲霉产生的红曲色素能较好地积累,总色价可达93.66U·mL-1。更多还原

【Abstract】 In order to take Monascus separated and purified from Red Kojic Rice as a stain,make the liquid state fermentation,and produce Monascus Pigment,taking five different sorts of carbon source,nitrogen source,pH and the volume of liquid as single factor,the three better standards among these factors via the pigment value and the determination of the thalli’s dry weight were determined,and finally make an orthogonal test.The orthogonal test showed the best condition of fermentation technology were 3% corn flour as carbon source,0.02% yeast extract as nitrogen source,adjusting the initial pH 4.0of fermentation broth and the volume of liquid was 125mL/250 mL,during Monascus Pigment produced by Monascus it would have a better accumulation and its color value would reach 93.66U·mL-1 in total.更多还原