【摘要】 以模式植物拟南芥(Arabidopsis thaliana)和专食性害虫小菜蛾(Plutella xylostella)为研究系统,运用蛋白质组学方法分析了拟南芥在小菜蛾取食诱导条件下叶片蛋白的差异表达情况。结果表明:有52个蛋白点在小菜蛾取食诱导条件下发生显著变化,其中28个蛋白点表达量上调,24个蛋白点表达量下调。运用质谱技术对其中的部分差异蛋白进行了鉴定,发现富含甘氨酸的RNA结合蛋白,3-磷酸甘油醛脱氢酶,ATP合酶的α亚基和β亚基在植物诱导防御中具有重要的作用,为揭示植物在昆虫取食条件下的化学防御机制提供蛋白质水平方面的理论基础。更多还原

【Abstract】 The differential expressed proteins of Arabidopsis thaliana that fed by the special diets insect Plutella xylostella compared with the normal growth were analyzed by use of the two dimensional electrophoresis. The results of the intensities of 52 protein spots in more than 650 spots were detected on each 2D gel changed significantly by using Image MasterTM2 D platinum 6.0 software.Among them,28 protein spots were up-regulated,and 24 spots were down-regulated under the insect feeding for 8 h. Some proteins were identified by MALDI-TOF-TOF MS successfully,such as the Glycine-rich RNA-binding protein,Glyceraldehyde-3-phosphate dehydrogenase,ATP synthase α and β subunits. These proteins might have played an important role in the process,and the further analysis study of the different proteins could offere the theoretical foundation to reveal the plant defense mechanism.更多还原