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添加VB1后安神补脑液对神经细胞的保护作用

The Protective Effect of Anshenbunao oral liquid Added VB1 on Nerve Cells

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【作者】 王婷婷孙桂波孟祥宝林文彬卢珊祖双王永宽于江波孙晓波

【Author】 Wang Tingting;Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Commerce;

【机构】 哈尔滨商业大学生命科学与环境科学研究中心中国医学科学院北京协和医学院药用植物研究所吉林敖东延边药业股份有限公司

【摘要】 目的:通过研究含VB1的全方安神补脑液、不含VB1的安神补脑液、VB1液三种药液对H2O2诱导损伤PC12细胞的保护作用,探讨添加VB1后的全方安神补脑液对神经的保护作用。方法:利用H2O2诱导损伤PC12神经细胞,通过MTT法检测细胞存活率,试剂盒检测乳酸脱氢酶(LDH)的漏出量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量,观察安神补脑液对PC12神经细胞损伤的保护作用。结果:三种药液对PC12神经细胞存活率均无显著影响,对H2O2诱导损伤的PC12细胞均具有保护作用。添加VB1后的全方安神补脑液对H2O2诱导PC12细胞损伤的保护作用优于不含VB1的安神补脑液及VB1药液。结论:添加VB1后的全方安神补脑液能增强其对神经细胞的保护作用。

【Abstract】 objective:The study observed the protective effect of An shen bu nao oral liquid with all components, An shen bu nao oral liquid excluded VB1 and VB1 oral liquid group against injury induced by H2O2 on PC12 cells and discussed the protective effect of An shen bu nao oral liquid added VB1 on PC12 cells. Methods:PC12 cells were cultured in vitro and injury was induced by H2O2.The survival rate of cells was detected by MTT.The contents of MDA,LDH and the level of SOD,CAT in cells were determined by kit respectively. Results:There are no obvious differences among the three groups in cell viability induced by H2O2. The protective effect of An shen bu nao oral liquid with all components on PC12 cells damaged by H2O2 was better than An shen bu nao oral liquid excluded VB1 and VB1 oral liquid group. Conclusion:An shen bunao oral liquid added VB1 has a protective effect on nerve cells which could enhance its neuroprotective effects

【基金】 国家科技重大新药创制课题(NO2012ZX09501001-004)
  • 【文献出处】 黑龙江医药 ,Heilongjiang Medicine Journal , 编辑部邮箱 ,2015年01期
  • 【分类号】R285
  • 【下载频次】131
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