【摘要】 目的探讨羟基酪醇和橄榄苦苷在氧化应激致猪LLC-PK₁（p LLC-PK1）细胞损伤中的拮抗作用。方法用10²00μmol/L过氧化氢处理pLLC-PK₁细胞,筛选最佳的处理时间与浓度,建立LLC-PK₁细胞过氧化氢氧化应激体外模型。LLC-PK1细胞分别与2、5和10μmol的羟基酪醇和橄榄苦苷孵育12 h,用100μmol/L的过氧化氢作用4 h,裂解LLC-PK₁细胞后,检测丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力和谷胱甘肽过氧化物酶（GSH-Px）活力和脂肪酸组成变化。结果与过氧化氢模型组（100μmol/L）相比,在橄榄苦苷和羟基酪醇处理的LLC-PK₁细胞中,MDA表达量降低,SOD和GSH-Px活力显著升高,脂肪酸转化降低,差异均有统计学意义（P<0.05,P<0.01）。结论羟基酪醇和橄榄苦苷对过氧化氢诱导的LLC-PK₁细胞氧化应激具有拮抗作用。更多还原

【Abstract】 Objective To explore the antagonism of hydroxytyrosol and oleuropein on porcine LLC-PK₁ cells caused by hydrogen peroxide（H₂O₂）. Methods The in vitro oxidative stress model of the LLC-PK₁ cells was established based on the opitimized time and concentration of the H₂O₂ treatment, cell viability was tested by MTT assay. After the LLC-PK₁ cells were incubated with 2 μmol/L, 5 μmol/L and 10 μmol/L of hydroxytyrosol and oleuropein for 12 h,respectively,100 μmol/L of H2O2 was added into the treated LLC-PK₁ cells,incubating for 4 h. After the LLC-PK₁ cells were lysed,the MDA content,SOD activity,GSH-Px activity,and fatty acid transformation were detected,respectively. Results Compared with H₂O₂ model group,in the LLC-PK₁ cell groups treated by hydroxytyrosol and oleuropein,the MDA content decreased significantly,the activities of both SOD and GSH-Px exhibited a significant increase,and the fatty acid transformation showed a significant decrease.Conclusion Hydroxytyrosol and oleuropein have a potential preventive effect on LLC-PK₁ cells suffering from the damage by H₂O₂ oxidative stress.更多还原