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拟南芥低光效突变体lpe1的筛选和特性分析
Screening and Characteristic Analysis of Low Photosynthetic Efficiency Mutant lpe1 in Arabidopsis thaliana
【摘要】 利用叶绿素荧光仪对化学诱变剂乙酰甲基磺酸(EMS)诱变的野生型拟南芥(Col-0生态型)F2代幼苗进行叶绿素荧光突变体筛选,获得了一株低光合效率突变体lpe1(low photosynthetic efficiency 1).遗传分析结果表明,lpe1是一个单基因隐性突变体.在生长发育的各个时期,lpe1叶片的叶绿素荧光值、叶绿素含量、淀粉含量和总蛋白含量明显低于野生型.
【Abstract】 The F2 population mutagenized by ethyl methanesulfonate(EMS)was screened by the chloroplast fluorescence imager and a low photosynthetic efficiency mutant lpe1 was screened in Arabidopsis thaliana(ecotype Col-0).Results of genetic analysis and physiological experiments showed that lpe1 is a single gene recessive mutant.Chlorophyll fluorescence,chlorophyll content,total protein content and starch content of lpe1 leaves were lower than those of the wild type at different growth stages.
【关键词】 拟南芥;
光合效率;
突变体;
筛选;
遗传分析;
生理分析;
【Key words】 Arabidopsis thaliana; photosynthetic efficiency; mutant; screen; genetic analysis; physiological analysis;
【Key words】 Arabidopsis thaliana; photosynthetic efficiency; mutant; screen; genetic analysis; physiological analysis;
【基金】 国家自然科学基金项目(U1404303);河南省基础与前沿技术研究项目(112300410219));河南省教育厅自然科学研究项目(2011A180023)
- 【文献出处】 河南大学学报(自然科学版) ,Journal of Henan University(Natural Science) , 编辑部邮箱 ,2015年06期
- 【分类号】Q945.11
- 【下载频次】78