【摘要】 目的观察红花黄色素对乳腺癌耐药细胞MCF-7/ADR缝隙连接蛋白Cx43的影响。方法取对数生长期的MCF-7/ADR细胞,以2×105cells/孔接种于6孔培养板,24 h后分为4组,每组3孔,其中3组分别加入红花黄色素至终浓度25、50、100 mg/L,另一孔设为空白对照组,作用48 h。Western blot法检测各组MCF-7/ADR细胞中Cx43表达水平。结果不同浓度红花黄色素作用于MCF-7/ADR细胞48 h后,Cx43表达水平均增高,有明显的剂量依赖关系,以50、100 mg/L浓度作用显著(P<0.01)。结论红花黄色素能提高乳腺癌耐药细胞MCF-7/ADR缝隙连接蛋白Cx43的表达水平。更多还原

【Abstract】 Objective To observe the effects of Safflower yellow on gap junction protein Cx43 in drug resistant breast cancer cell line MCF- 7 / ADR. Methods MCF- 7 / ADR cells of logarithmic growth phase were inoculated in six- well culture dishes( 2 × 105 cells each hole),and divided into 4 groups after 24 hours,3 holes in each group. 3 groups were added Safflower yellow to the final concentration of 25,50,100 mg / L,respectively. The last group was blank control group. 48 h after cultivating,the expression level of Cx43 in drug resistant breast cancer cell line MCF- 7 / ADR were detected by Western- blot. Results 48 h after different concentration of Safflower yellow acted on MCF- 7 / ADR cells,the expression level of Cx43 was increased in a dose- dependent relationship and there were remarkable effects on the concentration of 50,100 mg / L( P < 0. 01). Conclusion Safflower yellow can improve the expression level of gap junction protein Cx43 in drug resistant breast cancer cell line MCF- 7 / ADR.更多还原