【摘要】 目的探讨二十碳五烯酸(eicosapentaenoic acid,EPA)对增殖性糖尿病性视网膜病变(proliferative diabetic retinopathy,PDR)动物模型视网膜组织超微结构的影响。方法应用链脲佐菌素腹腔注射造成的糖尿病Sprague-Dawley大鼠模型,1个月后行血管内皮生长因子(vascular endothelial growth factor,VEGF)玻璃体腔注射造成PDR动物模型36只,在VEGF注射同时,注射后2、4周分别玻璃体注射EPA,并根据前期研究结果分为背景期(D组)12只、增殖前期(E组)8只、增殖期给药组(F组)4只,并与仅仅玻璃体腔注射VEGF的PDR模型组(C组)12只比较,应用透射电镜观察视网膜神经细胞的损害情况。结果电镜结果显示增殖前期给药组EPA注射后6周、背景期给药组EPA注射后4周视网膜神经细胞损害明显减轻。结论 EPA不仅可通过封闭flk-1受体抑制糖尿病性视网膜病变的发展,还对视网膜神经细胞具有保护作用,具体机制尚待进一步研究阐明。更多还原

【Abstract】 Objective To investigate the effect of eicosapentaenoic acid(EPA)on retinal ultrastructure of proliferative diabetic retinopathy(PDR)animal models.Methods Vascular endothelial growth factor(VEGF)was injected intravenously in 36 diabetic Sprague-Dawley rats at 1 month after receiving intraperitoneal injection of 60 mg/kg body mass of streptozotocin.Simultaneously,at 2,4 weeks after VEGF injection,EPA was injected intravenously in 12 rats as D,8 rats as E,4 rats as F group which represented treating group in background,pre-proliferation stage and proliferation stage of PDR based on early research results.The results were compared with 12 rats only received intraperitoneal injection of VEGF as C group.The damages of retinal neurocytes were observed by transmission electronic microscope.Results Transmission electronic microscope observation demonstrated the damages of retinal neurocytes relieved at 6 weeks after EPA injected in pre-proliferation stage eyes and 4 weeks after EPA injected in background stage eyes.Conclusion EPA can not only suppress development of diabetic retinopathy by blocking flk-1 receptor,but also protect retinal neurocyte whose mechanism is still waiting for approach.更多还原