【摘要】 目的建立耐甲氧西林金黄色葡萄球菌(methicillin resistant Staphylococcus aureus,MRSA)荧光定量PCR快速检测方法,筛查重症监护室(intensive care unit,ICU)MRSA定植者。方法采集患者感染部位标本或鼻咽拭子标本,快速提取总DNA后应用荧光定量PCR方法检测标本中nuc和mecA基因,对于双阳性结果报MRSA阳性,采取早期干预措施。结果建立的荧光定量PCR方法灵敏度高(4 CFU/反应)、快速、准确率100%。采取筛查和干预降低了ICU的MRSA感染率。结论应用荧光定量PCR对MRSA早期筛查及干预,有助于早发现、早诊断、早隔离、早治疗,可有效防止或终止MRSA导致的医院感染暴发流行。更多还原

【Abstract】 Objective To establish the fluorescent quantitative polymerase chain reaction(QPCR) method for screening methicillin resistant Staphylococcus aureus(MRSA) colonised patients of intensive care unit(ICU).Methods Collecting the infection specimen or nasal swabs,extracting the DNA,and detecting the nuc gene and mecA gene within the sample by Q-PCR.For the double positive results specimen,MRSA positive was reported and intervening measures were adopted.Results The Q-PCR method improved in this study was rather quick and showed high sensitivity(4 CFU/reaction) and accuracy(100%).Q-PCR screen and early infection control measures reduced the MRSA infection rate of ICU.Conclusion Early screening and intervening of MRSA are helpful to early detection,diagnosis,quarantine and treatment,and also can effectively prevent or stop hospital infection outbreak caused by MRSA.更多还原