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紫花苜蓿根响应盐胁迫的比较蛋白质组学分析
Comparative Proteomic Analysis of Salt-stress Response of Alfalfa Proteins in Root
【摘要】 6 d龄紫花苜蓿(Medicago sativa L.)幼苗在0、200 mmol/L Na Cl处理9 d后,采用双向电泳分别分离了其根部蛋白组。最终每块胶中有超过900个蛋白质点被分离,采用MALDI-TOF-TOF/MS质谱技术结合MASCOT软件在线检索,成功鉴定21个表达量发生1.5倍以上变化的蛋白质点,为19种不同的蛋白质。生物信息学分析表明,这些差异表达蛋白质主要参与胁迫防御、碳水化合物和能量代谢、次生代谢、转录和翻译调控、信号传导和离子转运等调控途径。
【Abstract】 6 day old alfalfa(Medicago sativa L.) seedlings were exposed to 0(control),and 200 mmol/L Na Cl for 9 days. Proteins extracted from the root of alfalfa seedlings were separated by two-dimensional gel electrophoresis(2-DE). More than 900 protein spots were detected on each gel,and 21 spots at least one point five-fold differences in abundance were identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry(MALDI-TOF-TOF/MS) and MASCOT online. Bioinformatics analysis induced that these proteins were associated with a variety of functions,including stress defense,carbohydrate and energy metabolism,secondary metabolism,transcription and translation related,signaling and ion transport.
【Key words】 alfalfa(Medicago sativa L.); salt-stress; root; proteomic;
- 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2015年21期
- 【分类号】S541.9
- 【被引频次】3
- 【下载频次】256