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紫花苜蓿根响应盐胁迫的比较蛋白质组学分析

Comparative Proteomic Analysis of Salt-stress Response of Alfalfa Proteins in Root

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【作者】 熊军波杨青川蔡化田宏张鹤山刘洋

【Author】 XIONG Jun-bo;YANG Qing-chuan;CAI Hua;TIAN Hong;ZHANG He-shan;LIU Yang;Institute of Animal Science, Hubei Academy of Agricultural Science;Institute of Animal Science of Beijing,Chinese Academy of Agricultural Science;

【机构】 湖北省农业科学院畜牧兽医研究所中国农业科学院北京畜牧兽医研究所

【摘要】 6 d龄紫花苜蓿(Medicago sativa L.)幼苗在0、200 mmol/L Na Cl处理9 d后,采用双向电泳分别分离了其根部蛋白组。最终每块胶中有超过900个蛋白质点被分离,采用MALDI-TOF-TOF/MS质谱技术结合MASCOT软件在线检索,成功鉴定21个表达量发生1.5倍以上变化的蛋白质点,为19种不同的蛋白质。生物信息学分析表明,这些差异表达蛋白质主要参与胁迫防御、碳水化合物和能量代谢、次生代谢、转录和翻译调控、信号传导和离子转运等调控途径。

【Abstract】 6 day old alfalfa(Medicago sativa L.) seedlings were exposed to 0(control),and 200 mmol/L Na Cl for 9 days. Proteins extracted from the root of alfalfa seedlings were separated by two-dimensional gel electrophoresis(2-DE). More than 900 protein spots were detected on each gel,and 21 spots at least one point five-fold differences in abundance were identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry(MALDI-TOF-TOF/MS) and MASCOT online. Bioinformatics analysis induced that these proteins were associated with a variety of functions,including stress defense,carbohydrate and energy metabolism,secondary metabolism,transcription and translation related,signaling and ion transport.

【基金】 国家自然科学基金项目(31402130;30871819)
  • 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2015年21期
  • 【分类号】S541.9
  • 【被引频次】3
  • 【下载频次】256
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