【摘要】 以二甲烯丙基色氨酸合成酶(DMATS)为研究对象,通过生物信息学方法共检索到37种微生物中的77条DMATS蛋白质序列。使用MEGA v4.0软件构建DMATS系统进化树发现,这些同源序列可分为两大簇,在第一簇中全为真菌的聚类,而第二簇中是细菌和真菌的聚类,初步分析可能是由于细菌和真菌的进化速度不同或DMATS基因是一个独立复制事件。在进行DMATS的信号肽结构预测时发现,只有Ajellomyces capsulata的DMATS(登录号:C6HC16)含有信号肽结构,由此可知,Ajellomyces capsulata(C6HC16)的DMATS可能是分泌蛋白质。通过使用MEME v4.9进行分析,最终得到了7条保守的motifs序列,这些motifs可能对DMATS发挥功能具有重要作用。更多还原

【Abstract】 Taking dimethylallyl tryptophan synthetase(DMATS) protein as the research object, a total of 77 DMATS protein sequences of 37 kinds of microorganism were retrieved by bioinformatics methods. The phylogeny analysis showed that these homologous sequences could be divided into two clusters, one containing only fungi, and the other containing both bacteria and fungi, which may be the result of different speed of evolution of bacterial and fungal or independent replication of DMATS genes. The DMATS of Ajellomyces capsulata(ID: C6HC16) was found to contain signal peptide by structure prediction, and it may be a secreted protein in Ajellomyces capsulata(ID: C6HC16). We finally got seven conserved motif sequences by MEME v4.9, which may have an important function in DMATS.更多还原