节点文献
基于psbA-trnH序列的剑麻H.11648原始母本的鉴定
Identification of Original Female Parents of Agave hybrid No.11648 Based on psbA-trnH
【摘要】 剑麻H.11648(Agave hybrid No.11648)是以蓝剑麻(A.amaniensis)和假菠萝麻(A.angustifolia)为亲本进行杂交,F1再与蓝剑麻回交选育而来。分析了剑麻H.11648及其两个亲本叶绿体psb A-trn H序列的差异。结果表明,剑麻H.11648与蓝剑麻的psb A-trn H存在1个明显的SNP差异,而在该位点与假菠萝麻保持一致,从叶绿体DNA水平证实了剑麻H.11648的细胞质来源于假菠萝麻。
【Abstract】 Agave hybrid No.11648(H.11648) was derived from the cross between A. amaniensis and A. angustifolia and backcrossed with A. amaniensis. The difference of psb A-trn H between H.11648 and its patents were analyzed by PCR amplification and sequencing. The sequence analysis showed a C/T SNP loci between the psb A-trn H of A. amaniensis and H.11648. The results indicated that the cytoplasm of H.11648 derived from A. angustifolia.
【关键词】 剑麻H.11648(Agave hybrid No.11648);
蓝剑麻(A.amaniensis);
假菠萝麻(A.angustifolia);
psbA-trnH;
【Key words】 Agave hybrid No.11648; A.amaniensis; A.angustifolia; psbA-trnH;
【Key words】 Agave hybrid No.11648; A.amaniensis; A.angustifolia; psbA-trnH;
【基金】 广西自然科学基金项目(2013GXNSFBA019116);国家现代农业产业技术体系建设项目(CARS-19-S15);广西壮族自治区直属公益性科研院所基本科研业务费专项资金项目(桂热研201206)
- 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2015年10期
- 【分类号】S563.8
- 【下载频次】48