【摘要】 利用生物信息学软件对Henan-09-03株乙型脑炎病毒(JEV)病毒E蛋白(囊膜蛋白)进行生物信息学分析、预测,为研究JEV的E蛋白基因工程疫苗提供依据。结果表明:E蛋白的分子质量为53 k Da,等电点是7.75,消光系数是66 515,不稳定系数为27.05,疏水平均值为-0.007,无信号肽,有2个跨膜区、27个蛋白修饰位点、5个二硫键、15个蛋白质结合位点,二级结构为α-β混合型、三级结构以同源四聚体形式存在。更多还原

【Abstract】 Bioinformatics of envelope protein of Japanese encephalitis virus Henan-09-03 strain was analyzed and predicted by information biology software for researching new type genetic engineering vaccine. The results showed that the molecular weight of envelope protein was 53 k Da,isoelectric point was 7. 75,extinction coefficient was 66 515,instability index was 27. 05,grand average of hydropathicity was-0. 007. It had no signal peptide,( two transmembrane domains,twenty-seven protein modification sites,five disulfide bonds,fifteen protein binding sites.Its secondary structure was alpha-beta mixed type,and tertiary structure was homotetramer.更多还原