【摘要】 为了探明板栗及其近缘种的亲缘关系并分析栗属的遗传多样性,基于叶绿体微卫星标记技术,选用4对呈现多态性的cp SSR引物,对板栗及其近缘种、野生种共6个种,56份材料进行遗传结构、遗传关系等分析。4个位点扩增等位基因数(Na)平均为3.25,有效等位基因数(Ne)平均为2.554,期望杂合度(He)平均为0.606,群体Nei’s遗传多样性(Hs)为0.320,各遗传参数值均低于核基因组对群体研究的相应值。另外,各种之间有丰富的cp SSR多样性,尤以野生板栗多样性指数最高。结果表明,我国天然野生板栗群体内蕴含更丰富的遗传变异,为我国板栗野生种质保育及可持续开发利用提供了基础数据和科学依据。更多还原

【Abstract】 The objective of the study is to elucidate the genetic relationship and genetic diversity of Castanea species. Based on the technology of chloroplast microsatellite markers,of total 24 cp SSR primer pairs screened,only four presented length polymorphism in the Castanea mollissima tested. We surveyed 6 species,altogether 56 materials with cp SSR markers in order to estimate genetic variation and genetic structure. In the castanea plants,average number of alleles( Na),effective number of alleles( Ne),expected heterozygosity( He) and Nei’ s were 3. 25,2. 554,0. 606 and 0. 320,respectively. Apparently,the parameters of cp SSR loci were much lower than those revealed by nuclear SSRs. Furthermore,the intra-specific genetic diversity was high but varied greatly among different species,the wild chestnut in China showed the highest genetic diversity,which one was rich in heredity and variation. The results have important implications of understanding the population genetics and evolutionary patterns in the genus Castanea and provide baseline data for formulating the conservation and management strategies.更多还原