【摘要】 为了深入研究嵴病毒(sw Ko V)主要结构蛋白基因VP1,根据Gen Bank中已发表的猪嵴病基因序列设计特异性引物,采用RT-PCR方法扩增猪嵴病毒CH441株VP1基因,并对其进行克隆与测序分析。结果表明,sw Ko V CH441株的VP1基因为762 bp,与Gen Bank已发表的嵴病毒属的15株嵴病毒序列的VP1基因相比较,sw Ko V CH441株的VP1基因与其他各毒株VP1基因的核苷酸同源性为81.5%~90.2%,氨基酸同源性为86.6%~96.9%,进化分析显示,sw Ko V CH441株与GS-1株之间的亲缘关系较近。生物信息学分析显示,VP1蛋白理论等电点(p I)为4.40,理论分子质量为26.978 2 k Da;其序列上共发现18个磷酸化位点,分别为Ser(7)、Thr(6)和Tyr(5),而蛋白的磷酸化与信号转导有关,预测该蛋白为一重要的信号转导分子;无信号肽和跨膜区。为进一步开展sw Ko V CH441株VP1基因在遗传变异等方面的研究奠定了理论基础。更多还原

【Abstract】 The aim of the study to investigate the main structural protein of the Kobuvirus VP1 gene. According to the sequences of PKV deposited in Gen Bank,a pair of special primers was designed for amplifying the VP1 gene of sw Ko V CH441 strain by RT-PCR. The results of sequence analysis showed that the whole VP1 gene of sw Ko V CH441 strain consisted of 762 bp. Compared with 15 PKV strains which were deposited in Gen Bank,the homology of nucleotide sequences was 81. 5% ~ 90. 2%,and the homology of deduced amino acids was 86. 6% ~ 96. 9%. Evolution analysis indicated that the sw Ko V CH441 strain was closely related to GS-1 strains. The bioinformatics analysis demonstrated that the isoelectric point and molecular weight of non-structural protein VP1 were 4. 40 and26. 978 2 k Da. The protein had no signal peptide and transmembrane domain. There were 18 phosphorylation sites including 7 Sers,6 Thrs and 5 Tyrs. Protein phosphorylation was concerned with signal transduction,so this protein may be a signaling molecule. The results provided a theoretical foundation for further research on the study of VP1gene( protein) in the genetic variation.更多还原