【摘要】 目的建立复方苦参败酱草保留灌肠剂中齐墩果酸的含量测定方法。方法采用高效液相色谱法,色谱柱为phenomenex lune(4.6×250mm,5μm),流动相为乙睛-水(90∶10),流速为1.0m L·min-1,检测波长为210nm,柱温25℃。结果齐墩果酸的检测线性范围为20~400μg·m L-1,r=0.9998,RSD为0.46%(n=5),平均回收率为100.34%。结论本法简便、准确、灵敏度高、重复性好,可作为复方苦参败酱草保留灌肠剂中齐墩果酸的含量测定方法。更多还原

【Abstract】 OBJECTIVE To establish a method for the determination of Oleanic Acid in compound sophora Herba Patrinia Retention enema. METHODS HPLC was used with phenomenex lune( 4. 6 × 250 mm,5μm) column,the mobile phase was acetonitrile-water( 90∶ 10). The flow rate was 1. 0m L·min- 1. The detection wavelength was 210 nm and column temperature was 25℃. RESULTS The range of inear detection of Oleanic acid was 20 ~400 gm L-1( r = 0. 9995). The average recovery was 99. 6%,RSD 0. 43%( n = 6). CONCLUSION The method is simple,accurate,highly sensitive and well represented. It can be used for routine quality control of compound Sophora Herba Patriniae Retention enema.更多还原