【摘要】 目的观察表没食子儿茶素没食子酸酯(EGCG)对柔红霉素(DNR)所致心肌损伤小鼠抗氧化作用的影响。方法昆明种小鼠随机分为空白对照组、模型组、EGCG高剂量组(80 mg/kg)、EGCG低剂量组(40 mg/kg)。空白对照组和模型组小鼠灌胃给予等体积生理盐水。给药7 d后,除空白对照组外,其余各组小鼠腹腔注射DNR(15 mg/kg)。48 h后取血,观察各组小鼠的血清和心肌组织的超氧化物歧化酶(SOD)、丙二醛(MDA)。结果模型组小鼠血清MDA含量显著高于空白对照组(P<0.05),SOD活性明显低于空白对照组(P<0.05)。EGCG高、低剂量组血清MDA含量显著低于模型组(P<0.05),EGCG高、低剂量组血清SOD活性则显著高于模型组(P<0.05)。模型组小鼠心肌MDA含量显著高于空白对照组(P<0.01),SOD活性明显降低(P<0.05)。EGCG高、低剂量组心肌MDA含量显著低于模型组(P<0.05),EGCG高剂量组心肌SOD活性显著高于模型组(P<0.05)。结论 EGCG对柔红霉素诱导的小鼠心肌损伤有一定的抗氧化作用。更多还原

【Abstract】 Objective To investigate the antioxidation effects of epigallocatechin gallate( EGCG) on daunorubicin( DNR)-induced myocardial injury in mice. Methods The Kunming mice were randomly divided into blank control group,model group,high dose EGCG group( 80 mg / kg),low dose EGCG group( 40 mg / kg). The mice in the blank control group and model group were given equal volume of saline by lavage. Seven days after treatment,the mice in each group were injected with DNR at a dose of 15 mg / kg except in blank control group. Forty-eight hours later,the superoxide dismutase( SOD) activity and malondialdehyde( MDA) content in serum and myocardium of mice were detected in each group. Results The serum MDA content of mice in the model group was significantly higher than that in the blank control group( P < 0. 05),and the SOD activity of mice in the model group was significantly lower than that in the blank control group( P <0. 05). The serum MDA content of mice in the high and low dose EGCG groups was significantly lower than that in the model group( P <0. 05),and the serum SOD activity of mice in the high and low dose EGCG groups was significantly higher than that in the model group( P <0. 05). The myocardial MDA content of mice in the model group was significantly higher than that in the blank control group( P <0. 01),and the SOD activity decreased significantly in the model group( P < 0. 05). The myocardial MDA content of mice in the high and low dose EGCG groups was significantly lower than that in the model group( P < 0. 05),and the myocardial SOD activity of mice in the high dose EGCG group was significantly higher than that in the model group( P < 0. 05). Conclusion EGCG plays an antioxidation role in the prevention of DNR-induced myocardial injury in mice.更多还原