【摘要】 目的:探讨建立弓形虫生殖毒性妊娠期小鼠动物模型的方法。方法:将妊娠期ICR小鼠随机分为孕早期感染组、孕晚期感染组及相应对照组,孕早期和孕晚期感染组小鼠再分别分为3小组,分别以灌胃方式感染弓形虫PRU株包囊10、20及40个,对照组灌胃生理盐水,观察各组小鼠不良妊娠结局及产仔数量。结果:弓形虫PRU株感染数量为10个包囊时,孕早期感染组孕鼠的死亡率为36.36%,出现阴道流血、流产、早产等现象,不良妊娠结局高达85.71%,与对照组比较差异有统计学意义(P<0.05);感染20包囊和40包囊时,孕早期感染组孕鼠的死亡率高达50%和100%;孕晚期感染组孕鼠在妊娠期发病不明显,产仔鼠数量较对照组减少,但差异无统计学意义(P>0.05);结论:用10个PRU株包囊灌胃能建立妊娠期弓形虫感染小鼠动物模型,为研究弓形虫PRU株所致生殖毒性奠定基础。更多还原

【Abstract】 Objective: To establish a suitable pregnant mice infection model with Prugniaud( PRU)strain of Toxoplasma gondii. Methods: ICR mice were randomly divided into early-pregnancy infection group,late-pregnancy infection group and the control group,and mices of each infection group were divided into 3 groups and were orally infected with 10,20 and 40 PRU strain cysts respectively,control group was given normal saline instead. Adverse pregnancy outcomes and litter amount were observed. Results: When infected with 10 PRU strain cysts,mortality of early-pregnancy mice was36. 6%,and vaginal bleeding,abortion,premature phenomena appeared,the incidence of adverse pregnancy outcomes reached to 85. 71%,there were significant differences compared with control group( P < 0. 05). Mortality of early-pregnancy mice was 50% and 100% respectively when infected with 20 cyst and 40 cyst. The morbidity of mice in late-pregnancy infection group was low,and litter amount reduced,but compared with the control group,there was no significant difference( P > 0. 05).Conclusions: Pregnant mice infection model is established with 10 PRU strain cysts of Toxoplasma gondii,which lays the foundation for the further study of reproductive toxicity of PRU strain.更多还原