【摘要】 目的:研究三阴乳腺癌(TNBC)MDA-MB-231细胞中雌二醇对整合素(integrin)β4水解的促进作用。方法:常规培养MDA-MB-231细胞至指数增长期,分别给予17β-雌二醇、雌激素受体(ER)特异性阻断剂4-羟基他莫西芬(OHT)和G蛋白偶联受体30(GPR30)特异性阻断剂G-15干预30 min,采用蛋白质印迹(Western blot)实验检测钙激活中性蛋白酶2(calpain 2)的表达和integrinβ4水解的情况。结果:17β-estradiol在短时间内可促进MDA-MB-231细胞130和95k D integrinβ4水解片段的产生,促进作用呈浓度依赖方式,而对calpain 2表达没有明显影响;4-OHT不能阻断17β-estradiol对integrinβ4的水解作用,且其本身可促进integrinβ4水解;G-15能阻断17β-estradiol对integrinβ4的水解。结论:在TNBC MDA-MB-231细胞中,17β-estradiol可通过GPR30促进130和95k Dintegrinβ4水解片段的产生。更多还原

【Abstract】 Objective: To investigate the way of estrogen to enhance the proteolysis of integrin β4 in triple negative breast cancer( TNBC) cell,MDA-MB-231. Methods: MDA-MB-231 was treated with17β-estradiol,4-hydroxytamoxifen( OHT) and G-15 for 30 min respectively or jointly when the cell reached to exponential growth stage. Then,Western blot was used to detect the proteolysis of integrinβ4 and the expression of calpain 2. Results: As Western blot results showing,not the expression of calpain 2,but the hydrolysis products of integrin β4 with molecular weight of 130 k D and 95 k D were raised by 17β-estradiol in a short time with dose-dependent way. However,an estrogen receptor( ER)specific inhibitor could enhance,instead of block,the proteolysis function of 17β-estradiol to integrinβ4,which could be blocked by the specific inhibitor of G-coupled protein receptor( GPR) 30,G-15.Conclusion: In TNBC cell,MDA-MB-231,the hydrolysis products of integrin β4 with 130 and 95 k D are enhanced by 17β-estradiol via GPR30.更多还原