【摘要】 以人工驯化的黄绿蜜环菌菌种为材料,在优化的培养基组成及培养温度下进行培养。通过单因素实验,考察不同的摇瓶装量、搅拌速度、发酵时间和接种量对黄绿蜜环菌菌丝体产量的影响,在此基础上通过正交设计确定最优深层发酵工艺参数,并通过小试和中试对其进行验证,黄绿蜜环菌最优化发酵工艺参数:摇瓶装量为150 m L/500 m L、搅拌速度为125 r/min、发酵时间为10 d、接种量为15%,此工艺可应用于工业化生产。更多还原

【Abstract】 A domesticated strain of Armillaria luteo-rivens was cultivated under the conditions of optimized medium composition and culture temperature. Through the single factor experiment,the effects of medium volume,stirring rate,fermentation period and inoculum size on mycelia yield were investigated. On the basis of single factor experiment,the optimum parameters of submerged fermentation were determined by using orthogonal design,and then were verified on laboratory scale and pilot scale. The optimized parameters of fermentation were as follows: loading volume 150 m L /500 m L,stirring speed 125 r / min,fermentation period 10 d,inoculum size 15%. The result indicated that this technology could be applied to industrial production.更多还原